However, turning off normalization can result in an increase in false positives. For example when a genome has multiple copies of a gene, but a SNP exists in only one of those copies. Sometimes, clear heterozygotes may be missed if the combined peaks are significantly lower than would be expected in that area of the chromatogram. There is a new checkbox to suppress the default normalization that the heterozygote analysis algorithm uses to better identify potential heterozygotes. Save time when assembling multiple sets of reads Heterozygote Analysis Enhancements Powerful but simple pattern matching automatically categorises reads into subprojects. A great time saver if you do a lot of related small sequencing projects using a well-defined naming convention. After assembly, contigs can be exported (to a variety of file formats, including fasta/fastq) retaining the project name in the contig names. A simple pattern-matching text box lets you define which characters in the input filenames should be treated as project names, and which should be treated as read names. Easily optimize codon usage for optimal expression in a different organism Automatic Assembly of Sub-projects with PhrapĪ new tab in the phrap parameters dialog lets you have MacVector automatically break out the input reads into sub-projects to be assembled separately. It will also show the user who made the modification, date, and how the sequence was before and after the action. When optimized a new Feature is annotated to the sequence showing that the CDS has been optimized, which algorithm was used and which codon usage table. A filter dialog after the algorithm runs lets you choose whether or not to apply the results to the original CDS, or to simply view the proposed changes. Simply select a CDS feature in the Map or Features tab of a nucleic acid sequence and choose Analyze | Optimize Codon Usage for CDS… The resulting dialog lets you choose the codon usage table (.bias file) to use, along with the genetic code and the optimization algorithm. There is a new Analyze function that will directly optimize codon usage of CDS features for enhanced expression in a different organism. Direct Codon Optimization of CDS Features MacVector 18.6 is a Universal Binary that will run natively on Apple Silicon Macs as well as existing Intel Macs. MacVector 18.6 has also been tested on the upcoming macOS release, macOS Sonoma and will be fully supported on it when Apple release it. It is supported on macOS High Sierra to macOS Ventura. MacVector 18.6 was developed and tested on macOS Ventura. MacVector 18.6 contains a wide variety of new or enhanced functionality, adding features such as one-click optimization of CDS coding regions, automatic phrap sub-project assembly of ABI reads based on naming, direct support of .csv and .tsv text files for Primer Database searching, optional inclusion of graphical information in GenBank exported sequence files and numerous tweaks and improvements to many different workflows. MacVector 18.6 (released July 2023) Overview
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |